

3D scientific illustration, single panel.A rod-shaped E. coli Nissle 1917 bacterium in translucent gray, cutaway view revealing a circular chromosome (green double helix). A magnified inset shows the lacZ locus replaced by two colored expression cassettes:• Blue cassette (secretory): PelB signal peptide (blue spiral) → A9R protein (blue ribbon) floating in periplasm with dotted arrows outward.• Orange cassette (surface-display): Lpp-OmpA anchor (orange helix) fused to B6R-M1R (orange globular domains) embedded in outer membrane, protruding outward.Above, CRISPR-Cas9 complex (scissors icon) and donor DNA fragment (striped) are depicted with arrow leading to integration.Text labels: “Chromosomal integration”, “No antibiotic markers”.Style: 3D render, realistic microbial cell, glowing edges, cinematic lighting, white background, scientific journal cover style.
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