

Scientific vector graphic, split into two vertical panels.Left panel: E. coli Nissle 1917 (gray rod), inside a blue plasmid labeled “ampR” with red warning icon. Text: “Not for clinical use”.Right panel: Same bacterium, no plasmid. Chromosome (green circle) with magnified inset showing lacZ locus replaced by cassette: three colored arrows – blue “P-A9R-T”, orange “P-LTB-B6R-T”, green “P-OmpA-M1R-T”. Flanked by dashed homology arms. Green checkmark.Below panels: small timeline – “CRISPR-Cas9 knockout → donor integration → sacB selection”.Clean vector style, white background, flat colors, high resolution, scientific publication style. No photorealism.
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