

A scientific illustration showing the design of an oral monkeypox vaccine using engineered Escherichia coli Nissle 1917 (EcN) with chromosomal integration of three monkeypox antigens (A9R, B6R, M1R). The image is split into two panels: left panel shows a plasmid with ampicillin resistance gene (crossed out, red “X”) and a note “Not for clinical use”; right panel shows the final vaccine strain with a bacterial chromosome (circular DNA) containing a knock-in expression cassette at the lacZ locus, no plasmid, no antibiotic markers. Below the bacteria, a schematic of the gastrointestinal tract with mucosal immune responses (IgA, IgG, T cells). Clean vector illustration style, bright colors, white background, high resolution, suitable for a scientific publication.
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