

A professional scientific research flowchart for a medical genetics paper. The theme is ‘MMACHC Gene Variant Analysis’. Icons include: DNA helix, Computer for Bioinformatics (HSF software), Plasmid vector (Minigene), Cell culture dish (HEK293T), Electrophoresis gel bands, and Western Blot protein bands. The colors should be professional blue and white. Steps: 1.Variant In-silico screening. 2.Minigene Vector construction. 3.Cellular Transfection. 4.Dual Analysis (RNA splicing and Protein expression).Please emphasize the dual-pathway from DNA mutation to RNA splicing and then to Protein expression, showing how they correlate with Early-onset and Late-onset clinical phenotypes.5.Clinical Phenotype Correlation. Arrows should connect the steps clearly. High quality, flat design, vector style."
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