

Flat 2D vector scientific mind map, BioRender-style, minimalistic, clean layout, white background. Mind map summarizing the experimental design for studying elevated Imidazole Propionate (ImP) levels on hypothalamic function. Central node: ImP. Branch 1: Elevated systemic ImP levels (Step 1: Systemic ImP administration and measurement). Branch 2: Hypothalamic gene expression changes (Step 2: RNA sequencing of hypothalamic tissue). Branch 3: Neuronal signaling alterations (Step 3: Changes in excitatory and inhibitory neurons, synaptic pathways). Branch 4: Behavioral assays (Step 4: Mouse behavioral tests for stress-related responses). Branch 5: Human validation (Step 5: Human brain imaging and ImP correlation). Clear arrows between steps, minimal labels. Emphasize ImP as central variable. Simplified icons only, minimal text, publication-ready. Negative prompt: radial layout, 3D, photorealistic, result-driven wording, excessive text
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