

Flat 2D vector scientific workflow diagram, BioRender-style, minimalistic, clean horizontal layout, white background. Linear experimental design workflow for a gut–brain axis study. Left to right steps: 1) Gut microbiome sampling and analysis to identify ImP-producing bacteria. 2) Measurement of ImP production and systemic levels in circulation. 3) Hypothalamic molecular and neuronal assessments following ImP exposure. 4) Behavioral assays in mouse models to assess stress-related responses. 5) Human cohort and brain imaging studies for translational validation. Use neutral, method-focused labels. Clear arrows between steps. Group mouse experiments as preclinical and human studies as validation. Simplified icons only, minimal text, publication-ready PPT figure. Negative prompt: radial layout, result-driven wording, highlighted outcomes, decorative style, 3D, photorealistic
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