

Scientific protocol flowchart, vector line art, blue ink on white, minimalist isometric style. Serpentine layout: 5 steps top row (left-to-right), 5 steps bottom row (right-to-left). Elements: 1.5ml Eppendorf tubes containing curled tissue scrolls with blue nuclei dots, P1000 pipette tips for liquid handling, visible liquid meniscus in tubes. Steps: 1) Transfer scrolls to tube; 2) Xylene (1ml), 10min; 3) Remove liquid; 4) 100% Ethanol (1ml), 5min; 5) Sequential repeats: 100%, 70%, 50% Ethanol (5min each); 6) Water (1ml), 5min; 7) 1×TE (1ml), 95°C 30min; 8) Remove TE. Annotations: "Repeat 2x" curved arrow (top), "Repeat sequentially" list box (bottom right), "Remove without breaking the scrolls" labels. Colors: Dark blue outlines (RGB 45,85,135), light blue liquid fills (RGB 180,200,220), sans-serif technical font, crisp gray arrows. Style: Clinical precision, Nature/Science journal figure aesthetic, clean white background, no shadows, no 3D effects.
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