简约科学图表风格实验室操作流程图肠道屏障功能测定方

请将以下步骤绘制成流程图Intestinal barrier function assayWorm embryos were exposed to PS-NP beads in complete K-mediumat a concentration of 15 μg/L for 7 days (resulting in day 4 adults). Subsequently, these worms were washed with complete K-medium andallowed to dry on NGM plates for 20 min. Approximately 10 worms werethen incubated at 20 ◦C for 3 h in a liquid culture containing 30 μL ofOP50 bacteria mixed with Blue No.1 food dye (FD&C) [29]. Followingthis incubation, each worm was transferred to an unseeded NGM plate. Each individual worm was placed on a 10% agar pad on a glass slide,with the addition of 2.5 μL of M9 buffer. Observations were conducted aLeica DM2000 compound microscope.